Tekniker

Protein patterning by thermal nanoimprint lithography and NH3-plasma functionalization of polystyrene.

Author: V. Trabadelo, A. Retolaza, S. Merino, A. Cruz, P. Heredia, A. Foelske, H. Schift, C. Padeste. Date2009

New approach to create a pattern of specific protein binding sites via nanoimprint lithography is presented. 

NH3-plasma functionalized, patterned polystyrene covalently binds a biotin linker to which streptavidin is bound. The streptavidin is used as a versatile anchor for biotinylated functional proteins by means of a sandwich-type quantitative fluorescence immunoassay. Rabbit IgG is the analyte used as a “proof of concept” for the designed system, yielding a detection limit of approximately 50 ng/ml.

This site uses cookies

We use cookies to improve the browsing experience and provide our services to you.

If you continue browsing, we consider that you accept their use.

If you wish to obtain additional information, please query our Cookies policy.

Accept